Chymotrypsin CAS NO.9004-07-3

1.USP /EP standard 2.Porcine or bovine pancreas 3.Assay: no less than 1000u/mg

Chymotrypsin

CAS 9004-07-3

Source Bovine (Swine) pancreas

General description

Chymotrypsin is also called chymotrypsinogen, which is the endopeptidase extracted and separated from the fresh pancreas of cows (or pigs).

Primary structure and spatial structure of α- Chymotrypsin have been clearly explained, which is a single peptide chain composed of 245 amino acid residues, with 5 pairs of disulfide linkage in the molecule. It is white and light yellow crystal or amorphous powder, and easily soluble in the water, but insoluble in organic solvent. Relative molecular mass is 24000, and the optimum PH is 8~9. It is stable at a dry state, but will be quickly deactivated in water solution, most stable in the water solution with PH3~4. When it acts on the protein, it will hydrolyze the peptide bond formed by the carboxyl of L-tyrosine and L-phenylalanine in priority.

The high purity Chymotrypsin of Beijing Geyuantianrun Bio-tech Co., Ltd.. is purified by re-crystallization, and then by Chromatography and ultra-filtration.

Specification

Items	Specification	Method
Appearance	White crystallized powder	USP33
Identification	No purple color develops within 3 minutes	USP33
Loss on drying	No more than 5.0%	USP33
Residue on Ignition	No more than 2.5%	USP33
Microbial limits
Pseudomonas aeruginosa	Absent	USP33
Salmonella	Absent	USP33
Staphylococcus aureus	Absent	USP33
Limit trypsin	Not more than 1%.	USP33
Assay	NLT 1000 USP Chymotrypsin Units/mg (calculated on the dried basis) and 90.0%-110.0% of the labeled potency.	USP33

Storage

 Sealed, Dark, at temperature 2-8°C

Items	Specification	Method
Description	white crystallize powder	EP6.0
Identification	A) A purple colour develops B)No colour develops with in 3 min of mixing	EP6.0
Appearance of solution	Solution S is not more opalescent than reference suspension 2	EP6.0
Absorbance	1) 281nm : 18.5 ~ 22.5 2) 250nm : not more than 8.0	EP6.0
PH	3.0-5.0	EP6.0
Histamine	Not more than 1µg/5.0µkatal	EP6.0
Loss on drying	Not more than 5%	EP6.0
Limit of trypsin	Not more than 1%	EP6.0
Assay	Not less than 5.0 ukatal/mg, calculated on the dried basis	EP6.0

Storage

 Sealed, Dark, at temperature 2-8°C

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